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KMID : 1161420200230101093
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Journal of Medicinal Food
2020 Volume.23 No. 10 p.1093 ~ p.1101
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Structural Characterization and In Vitro Cytokines Modulation Effect of New Acetylated Galactomannans from Aloe arborescens
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Nazeam Jilan A.
El-Hefnawy Hala M.
Singab Abdel-Naser B.
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Abstract
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Three new galactomannan components designed as AANP-1, AANP-3, and AAAP-7 were isolated from previously purified Aloe arborescence polysaccharide fractions, using ion-exchange, gel filtration, and preparative chromatographic techniques. Based on Fourier transform-infrared, one-dimensional and two-dimensional nuclear magnetic resonance spectroscopy, the main backbone of AANP-1 elucidated as (1?→?4)-linked_α-d-deoxyGalp, (1?→?4) d-Manp, and β-d-2-glup. The sugar residue sequence of AANP-3 was (1?→?4)-linked β-d-NHAC-GAlp with β-d-AcManp side chain that attached to O-4 position. The AAAP-7 repeated units were (1?→?4) d-Manp linked with (1?→?4) d-Galp interspersed with (1?→?3)-α-Manp. The results of high performance size-exclusion chromatography indicated that the approximate molecular weights of AANP-1, AANP-3, and AAAP-7 were 4.2, 2.4, and 2.5?kDa, respectively. The cytokine modulation assay revealed that the isolated components promoted a remarkable release of interleukin (IL)-12 and tumor necrosis factor alpha in comparison with the negative control group, whereas potent significant induction of IL-2 and interferon gamma was detected more than the positive control phytohemagglutinin (P?
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KEYWORD
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Aloe arborescens, cytokines, galactomannan, structural elucidation
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